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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Periodontal Res. 2017 Feb 18;52(4):725–733. doi: 10.1111/jre.12439

Figure 1. Oral bacteria load, serum total IgG measurement and animal weight monitoring.

Figure 1

(A) On Day 0 and Day 20, DNA was extracted from oral swabs taken from each mouse and was quantitated by real-time PCR. A universal 16S rRNA gene primer was used to quantitate the total oral bacteria recovered from each animal. The number of bacteria count was significantly increased when OF was applied (Group 2 vs. Group 1), whereas the number of bacteria count was significantly decreased when additional SA was administered (Group 3 vs. Group 2). (B) Total serum IgG levels on Day 0 and Day 20 in all three groups of mice were quantitated by ELISA. Serum total IgG levels were significantly higher in Groups 2 and 3 as compared to those in Group 1. However, no difference was observed between Group 2 and Group 3. (C) The weight of mice was monitored throughout the experimental period on Days 0, 13, and 20. A stable body weight was maintained by all the three groups of mice. *p < 0.05. n = 8.