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. 2005 Feb 24;33(4):1169–1181. doi: 10.1093/nar/gki251

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© The Author 2005. Published by Oxford University Press. All rights reserved

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The effect of C0 initiation codon removal on expression at downstream initiation. (A) Schematic diagram of the HBV test constructs used to study the effect of C0 on downstream expression. The initiation codon with a darker font is in-frame to the luciferase reporter gene and an asterisk (*) indicates the position where a mutation is introduced. (B) Result of DNA transfection of the above constructs into HepG2 cells. The normalized luciferase activity of each construct with C0 mutation is compared with its respective parent construct without the C0 mutation. Normalized expression levels are presented as a percentage of the CLUC construct, assigned 100%. These results are averages of replicates from two independent experiments. Results from the C, C1 and C2 series represent sums of the activities of fusion proteins with similar specific activities.