Table 1.

PCR primers used to generate parent constructs

Primers	Sequence
APH3T7F	5′-gggcccaaAAGCTTTAATACGACTCACTATAGG-3′
H3T7PCRNAF	5′-gcgAAGCTTTAATACGACTCACTATAGGTAGGCACAAATTGGTCTGCG-3′
H3T7PGRNAF	5′-gcgatAAGCTTTAATACGACTCACTATAGGAACTTTTTCACCTCTGCCTAATC-3′
AatIILUCR	5′-gtttttggcgtcttcGACGTCgggcatTTGGTGGTCTATAGG-3′
XbaIF	5′-gcTCTAGAAACTTTTTCACCTCTGCCTAATC-3′
XbaIR	5′-gcTCTAGAATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAGCTCCAAATTCTTT-3′
GLR	5′-CCAGGGCGTATCTCTTCATAGCC-3′

The italicized sequence represents the T7 promoter and the underlined sequences represent the restriction enzyme site.