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MyoD gene knockout in QM7 cells. (A) Genomic sequence and structure of the quail MyoD gene. (B) Expression vector of Cas9-GFP and MyoD guide RNA (gRNA). The U6 promoter controls gRNA transcription followed by a termination signal. (C) Experimental overview and fluorescence-activated cell sorting (FACS) for the development of single cell-derived MyoD knockout QM7 sublines.
