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. 2016 Oct 28;30(7):1029–1036. doi: 10.5713/ajas.16.0749

Figure 4.

Figure 4

(A) Nuclear fusion in regular QM7 (rQM7) and MyoD-knockout QM7 (MyoD KO QM7#4) cells during myotube differentiation. No fusion was evident in MyoD KO QM7#4 cells even after 6 days of differentiation. (B) Western blotting detected MyoD protein in rQM7 and MyoD KO QM7#4 cells after 3 days of differentiation. (C) RT-PCR analysis of myogenic differentiation-related genes and quantitative RT-PCR of the MyoD gene in rQM7 and MyoD KO QM7#4 cells during differentiation. (D) Detection of myogenic differentiation-related genes in rQM7 and MyoD KO QM7#4 cells during differentiation. RT-PCR, reverse transcription-polymerase chain reaction. The images were observed under the inverted fluorescent microscope after staining with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI), scale bars = 200 μm.