Table 1.
Gene | Forward | Reverse | Annealing temp. (°C) |
---|---|---|---|
GAPDH | TGTCAAGGCTGAGAACGGGA | AGGCCAGGGAGCATTCTTCT | 60 |
MyoD | ATTGGCAATGAGAGGTTCAGG | TAGAGCCTCCAATCCAGACAGA | 60 |
Myogenin | AGCCTCAACCAGCAGGAGC | TGCGCCAGCTCAGTTTTGGA | 60 |
Desmin | CTGAAGGATGAGATGGCC | GGTCGCCTCGCTCACCAC | 60 |
GAPDH, glyceraldehyde 3-phosphate dehydrogenase.