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. 2005 Feb 24;33(4):1193–1200. doi: 10.1093/nar/gki263

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© The Author 2005. Published by Oxford University Press. All rights reserved

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RecA-catalyzed strand transfer. The upper panel shows a schematic diagram of the reaction. Single-strand (SS) circular DNA reacts with linear (L) duplex to form intermediate (I) structures which are converted into nicked-circle (NC) products. The fluorograph shows the results from the homologous M13–M13 (lanes a–e) and the homeologous M13-fd (lanes f–j) reactions. Samples were removed at time 0 (lanes a and f), 5 min (lanes b and g), 15 min (lanes c and h), 45 min (lanes d and i) and 90 min (lanes e and j).