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. 2005 Feb 24;33(4):1193–1200. doi: 10.1093/nar/gki263

Table 2.

Yield of recombinants in homologous and homeologous crosses

Donor Plasmid in recipient Recipient Selected marker(s) Frequency Fold increase
AB259 mutS+ Rec+ Thr+ Leu+ 2.8 × 105
AB259 mutSΔ680 Rec+ Thr+ Leu+ 2.5 × 105 1
AB259 mutSΔ800 Rec+ Thr+ Leu+ 2.0 × 105 1
SA536 mutS+ Rec+ Arg+ 120 1
SA536 mutSΔ680 Rec+ Arg+ 59 000 492
SA536 mutSΔ800 Rec+ Arg+ 34 000 283
SA977 mutS+ Rec+ Arg+ 35 1
SA977 mutSΔ680 Rec+ Arg+ 10 665 296
SA977 mutSΔ800 Rec+ Arg+ 3410 100
SA536 mutS+ recA56 Arg+ 30 1
SA536 mutSΔ680 recA56 Arg+ 80 2.7
SA536 mutSΔ800 recA56 Arg+ 60 2
SA977 mutS+ recA56 Arg+ 8 1
SA977 mutSΔ680 recA56 Arg+ 120 15
SA977 mutSΔ800 recA56 Arg+ 85 10

Donor and recipient (GM4799 and GM5550) cultures (at 1–2 × 108 cells per ml) were mated for 60 min (AB259) or 3 h (SA strains). The numbers in the table represent the Thr+ Leu+ [StrR] or Arg+ [StrR] recombinants in 50 μl of undiluted mating mixture.