Table 2.
Yield of recombinants in homologous and homeologous crosses
Donor | Plasmid in recipient | Recipient | Selected marker(s) | Frequency | Fold increase |
---|---|---|---|---|---|
AB259 | mutS+ | Rec+ | Thr+ Leu+ | 2.8 × 105 | — |
AB259 | mutSΔ680 | Rec+ | Thr+ Leu+ | 2.5 × 105 | 1 |
AB259 | mutSΔ800 | Rec+ | Thr+ Leu+ | 2.0 × 105 | 1 |
SA536 | mutS+ | Rec+ | Arg+ | 120 | 1 |
SA536 | mutSΔ680 | Rec+ | Arg+ | 59 000 | 492 |
SA536 | mutSΔ800 | Rec+ | Arg+ | 34 000 | 283 |
SA977 | mutS+ | Rec+ | Arg+ | 35 | 1 |
SA977 | mutSΔ680 | Rec+ | Arg+ | 10 665 | 296 |
SA977 | mutSΔ800 | Rec+ | Arg+ | 3410 | 100 |
SA536 | mutS+ | recA56 | Arg+ | 30 | 1 |
SA536 | mutSΔ680 | recA56 | Arg+ | 80 | 2.7 |
SA536 | mutSΔ800 | recA56 | Arg+ | 60 | 2 |
SA977 | mutS+ | recA56 | Arg+ | 8 | 1 |
SA977 | mutSΔ680 | recA56 | Arg+ | 120 | 15 |
SA977 | mutSΔ800 | recA56 | Arg+ | 85 | 10 |
Donor and recipient (GM4799 and GM5550) cultures (at 1–2 × 108 cells per ml) were mated for 60 min (AB259) or 3 h (SA strains). The numbers in the table represent the Thr+ Leu+ [StrR] or Arg+ [StrR] recombinants in 50 μl of undiluted mating mixture.