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. 2005 Feb 24;33(4):1240–1248. doi: 10.1093/nar/gki278

Figure 3.

Figure 3

Characterization of SOFA+-δRz-303. (A) Autoradiogram of a denaturing 6% PAGE gel showing the cleavage assays of the HBV-derived substrate by SOFA+-δRz-303 bearing biosensor stems of various lengths (BS-X, where X indicates the length of the stem). The reactions were performed under single turnover conditions at 37°C for 3 h. (B) Autoradiogram of a 20% PAGE gel showing the cleavage assays of the 44 nt HBV-derived substrate by a SOFA+-δRz-303 bearing a biosensor stem of various lengths. The reactions were performed at 37°C for 2 h under multiple turnover conditions. XC indicates the position of the xylene cyanol dye. For (A) and (B), the length of the bands in nucleotides is shown adjacent to the gel. The control (−) was performed in the absence of ribozyme.