Table 1.

Kinetic analysis of various versions of SOFA-δRz-303 under single turnover conditions

Ribozymes	kcat (min−1)	KM (nM)	kcat/Km (min−1 M−1)
δRz-303	0.007 ± 0.001	750 ± 100	9.3 × 103
SOFA+-δRz-303-BS15	0.053 ± 0.008	226 ± 30	2.3 × 105
SOFA−-δRz-303-BS15	0.00008 ± 0.00002	600 ± 150	1.3 × 102
SOFA+-δRz-303-BS10	0.023 ± 0.003	150 ± 20	1.5 × 105
SOFA−-δRz-303-BS10	0.00004 ± 0.00001	600 ± 150	6.7 × 101