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. 2017 Jul 3;7:4546. doi: 10.1038/s41598-017-04874-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HSP90 is increased in PAH-PASMCs and stabilizes HDAC6 expression. (A) HSP90 expression was assessed by Western blot in pulmonary artery smooth muscle cells (PASMCs) from control (n = 3) and PAH patients (n = 5) and distal PAs from control (n = 3), MCT (n = 3) and Su/Hx (n = 3) rats. Protein band densitometry is reported in the corresponding graph. (B) Expression of HDAC6, acetylated-α-Tubulin, and α-Tubulin was assessed in PAH-PASMCs by Western blot after treatment or not with a pharmacological HSP90 inhibitor (AT13387, 10–100 nM for 48 hours) or vehicle (dimethyl sulfoxide). (C) Expression of HSP90, HDAC6, acetylated-α-Tubulin, and α-Tubulin was assessed in PAH-PASMCs by Western blot after treatments with a siHSP90 or siSCRM (20–100 nM for 48 hours). Blots are representative of two independent experiments. *P < 0.05.