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. 2017 Jul 3;7:4510. doi: 10.1038/s41598-017-04601-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Isolation method of CTC. White arrows point at filter pores, black arrows at “CTC” grey arrow points at the metal rim of the filter. Examples of CTC in patients with PDAC. (b) CTC cluster (patient 24), the scale bar represents 50 µm C single CTC (patient 23) the scale bar represents 10 µm. Arrows point at CTC, arrowheads at filter pores.