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. 2017 Jul 3;7:4510. doi: 10.1038/s41598-017-04601-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Example Chromatogram (antisense strand) wild-type KRAS in a negative control sample. (b) Example Chromatogram (antisense strand) KRAS c.35G > A (pG12D) in a CTC sample. (c) Example ddPCR result (Patient 45) for KRAS ^G12D. The number of events is very low which represents a very low copy of mutant KRAS ^G12D molecules in the specimen. The circle encircles the area of interest and the approximately 6 events.