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. 2005 Feb 24;33(4):e39. doi: 10.1093/nar/gni039

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Reconstitution of IFN-γ responsiveness upon the expression of cDNA1.2. (A) Detection of pStat1 in unstimulated and IFN-γ-stimulated cells transduced with BMN-I-GFP and BMN-I-GFP-cDNA1.2, respectively. Numbers represent the percentage of cells within each quadrant. (B) Western-blot analysis of cell lysates of wt U937, naive U937 clone #10.13, BMN-I-GFP transduced #10.13 and BMN-I-GFP-cDNA1.2 transduced unstimulated cells (−) and after IFN-γ stimulation (+). Antibodies raised against Stat1 (upper panel) and pStat1 (pY701; lower panel).