Figure 9.

Apoptosis and necrosis induced by H²K4bT and H³K4bT polymers. MDA-MB-435 cells were transfected with polymers H³K4bT and H²K4bT in complex with a luciferase-expressing plasmid as detailed in Material and Methods; the treatment groups are untreated (A), H³K4bT (B) and H²K4bT (C). The cells were then treated with YO-PRO-1 and PI fluorescence dyes and analyzed by flow cytometry using 488 nm excitation. V, viable cells; A, apoptotic cells; and N, necrotic cells.