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. 2017 Jul 3;7:4534. doi: 10.1038/s41598-017-04890-4

Figure 1.

Figure 1

Murine BMMCs can develop ET. BMMCs were pretreated with LPS (300 ng/ml) for 24 h to induce ET. For analysis of the cytokine expression, the cells were stimulated with LPS (1 µg/ml) for 30 min and 60 min and the gene expression of Il6 (a) and Tnf (b) was measured. Data show mean ± SD from n = 3 independent experiments. Two-sided ANOVA with Tukey HSD post-hoc test and one-sample t-Test were performed to calculate the p-values. Multiple testing p-values were corrected by FDR. Furthermore, LPS-pretreated and naive cells were stimulated with LPS (1 µg/ml) for 4 h to analyse protein production of IL-6 (c) and TNF-α (d). Data show mean ± SD from three independent experiments. Student’s t-Test was performed to calculate the p-values. **p < 0.01, ***p < 0.001.