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. 2017 Jul 3;7:4534. doi: 10.1038/s41598-017-04890-4

Figure 3.

Figure 3

NFκB p65 and p50 binding to cytokine promoters is reduced in tolerant BMMCs. BMMCs were treated with the IKK-β inhibitor Inh IV (3 µM) for 1 h and then stimulated with LPS (300 ng/ml) for 4 h to measure IL-6 (a) and TNF-α (b) protein production. Data show mean ± SD from n = 3 independent experiments. Student’s t-Test was performed to calculate the p-values. To induce ET, BMMCs were incubated with LPS (300 ng/ml) for 24 h. Cells were left unstimulated (con) or stimulated with LPS (1 µg/ml) for 30 min and 60 min and ChIP analysis was performed to measure NFκB p65 (c) and NFκB p50 (d) binding to Il6 and Tnf promoters. Data show mean ± SD from three independent experiments. Two-sided ANOVA with Tukey HSD post-hoc test was performed to calculate the p-values. Multiple testing p-values were corrected by FDR. *p < 0.05, **p < 0.01, ***p < 0.001.