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. 2017 Jul 3;7:4534. doi: 10.1038/s41598-017-04890-4

Figure 5.

Figure 5

Antigen-triggered cytokine expression is not affected in tolerant BMMCs. To induce ET, BMMCs were treated with LPS (300 ng/ml) for 24 h and loaded with IgE (150 ng/ml) overnight. Tolerant and naive cells were stimulated with LPS (1 µg/ml) or Ag (DNP-HSA; 20 ng/ml) for 90 min and Il6 (a) and Tnf (b) gene expression was measured. Data show mean ± SD from three independent experiments. Student’s t-Test and one-sample t-Test were performed to calculate the p-values. Multiple testing p-values were corrected by FDR. Furthermore, naive and tolerant cells were incubated with LPS (1 µg/ml) or Ag (DNP-HSA; 20 ng/ml) for 4 h to analyse protein production of IL-6 (c) and TNF-α (d). Data show mean ± SD from n = 3 independent experiments. Student’s t-Test was performed to calculate the p-values. *p < 0.05, **p < 0.01, ***p < 0.001.