Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 3;7:4543. doi: 10.1038/s41598-017-04876-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Serum withdrawal does not modulate the effect of hyperosmolarity on autophagy in NP cells. (a) Western blot analysis of NP cells cultured in serum-free media with increasing osmolarity (330–600 mOsm/kg H₂O) showed that the levels of autophagic markers including LC3-II, ATG12-ATG5, and BECN1 were unaltered. SQSTM1 levels did not change with increasing osmolarity except for a small decrease at 600 mOsm/kg H₂O. (b,c) Densitometric analyses of multiple Western blots. Bars represent mean ± SEM (n = 5). One-way ANOVA with Sidak’s multiple comparisons test was used to determine statistical significance. NS, non-significant. Western blot images were cropped and acquired under same experimental conditions. See Supplementary Fig. S1 for examples of uncropped images.