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. 2017 Jul 3;7:4535. doi: 10.1038/s41598-017-04361-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transgenic lines that overexpress an LRR kinase are dwarfed. (a) Non-transgenic plants (left hand panel) are taller than transgenic plants (right hand panel). (b) Regression plot showing vein number versus leaf width for one individual from each of two independent T1 lines – 50.1 (orange spot) & 50.2 (blue spot). (c–e) Cropped gel images of genomic (c) and RT- (d,e) PCR reactions illustrate transgene presence and transcript levels in an individual from each T1 line. Scale bar = 5 cm.