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. 2017 Jul 3;7:4523. doi: 10.1038/s41598-017-04776-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localisation of DLX5, HOXA10 and TLX1 proteins in first and third trimester placentae Representative sections of n = 5 first trimester and n = 5 third trimester placentae. DLX5, HOXA10 and TLX1 are expressed in the syncytiotrophoblast layer (black arrows) of first trimester (A–C) and third trimester (D–F) placentae. CK7 was used as a positive marker (H) and IgG as a negative control (G). (I) Dot plot shows immunoreactive signal pixel density for DLX5, HOXA10 and TLX1 in first and third trimester villi. Intensity for all three candidate proteins was significantly lower in the third trimester. The intensity of staining was calculated using an AxioVision software (Carl Zeiss KS 400, Germany) from five fields of view of each placental section and the results were expressed as the mean pixel density (in arbitrary units). Str = stromal.