Fig. 2.

Antenatal Beta abolishes the inhibition of sodium uptake by the nitric oxide donor SNAP and cGMP analog 8-Br-cGMP in male but not female RPTC. Na⁺ uptake in RPTC from male (vehicle-treated, n = 6, top left; Beta, n = 8, bottom left) and female (vehicle-treated, n = 4, top right; Beta, n = 9, bottom right) animals was expressed as area under the curve (AUC), plotted as those shown in Fig. 1. For these experiments, cells were incubated with Ang-(1–7) (A7; 1 pM), the NO donor (±)-S-nitroso-N-acetylpenicillamine (SNAP; 100 µM), or the cGMP stable analog 8-Br-cGMP (1 µM). Values are means ± SE. *P < 0.05 vs. untreated cells.