Expression levels of Ang-(1–7)-Mas receptor (Mas-R) in kidney from male and female vehicle and Beta-treated animals. Protein expression of the Ang-(1–7)-Mas receptor was measured by Western blot in kidney cortex isolated from male (A) and female (B) vehicle and Beta-exposed sheep. Signals for actin were obtained by reblotting of the same membrane; all samples were derived at the same time and processed in parallel. Densitometric analysis of gels is also shown for males (A; vehicle, □, n = 4; Beta, ■, n = 4) and females (B; vehicle, □, n = 4; Beta, ■, n = 4). Values are means ± SE. *P < 0.05 vs. vehicle. O.D., optical density.