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. 2017 Mar 29;312(6):F963–F970. doi: 10.1152/ajprenal.00078.2017

Fig. 4.

Fig. 4.

EGFR inhibitors increase exosome production and blocks wound healing. A: BUMPT were scratch-wounded with the device and then incubated with or without 1 μM gefitinib, and healing distance was recorded with a phase-contrast microscope. The wound width was measured at 6, 12, and 18 h after scratching to determine the healed distance. *P < 0.05 vs. gefitinib group. B: whole cell lysate was collected for immunoblot analysis of P-EGFR. C: densitometric analysis of P-EGFR expression. *P < 0.05 vs. control; #P < 0.05 vs. wound-only group. D: exosome was collected for immunoblot analysis; gefitinib treatment increased the expression of CD63 and TSG101 in exosomes after wounding. E: densitometric analysis of CD63 and TSG101 expression. *P < 0.05 vs. control group; #P < 0.05 vs. wound-only group. F: exosome number was measured by nanoparticle tracking analysis. *P < 0.05 vs. control; #P < 0.05 vs. wound-only group.