Fig. 1.

Sequence alignment of human and mouse anoctamin1 (Ano1) isoforms. The human Ano1 isoforms Ano1(0abcd) (19, 31), Ano1(abcd) (19, 31), hAno1(Δ1,2bcd) (19, 29), hAno1(Δ1,2,3bcd) (19), and hAno1(bcd) (29) were previously characterized by electrophysiology and are aligned using sequences from the current human annotation (#108). The mouse Ano1 sequence (annotation #106) is aligned to the human sequences using the transcript XM_006508458.3 that contains the commonly used full-length sequence. This alignment does not include the recent mouse transcript XM_006508463.3 (that has an alternative 5′-end with an alternative exon 1 that joins to exon 2 by skipping the current exon 1) that has not been previously studied by electrophysiology. In colors are highlighted the segments “a,” “b,” “c,” and “d” with the exon correspondence between mouse and human genes. The putative transmembrane domains are highlighted in gray.