Fig. 3.

Deactivating currents from the Ano1₍₀₎ΔEAVK splice variant have a higher EC₅₀ of Ca²⁺ dependence than Ano1₍₀₎. A: representative Cl⁻ current traces deactivating from a conditioning voltage step to +80 mV, recorded from HEK293 cells transfected with Ano1₍₀₎ (left) or Ano1₍₀₎ΔEAVK (right) and dialyzed with intracellular Ca²⁺ concentrations ([Ca²⁺]_i) ranging from 100 to 3,000 nM as indicated by the spectrum. Dotted line: 0 pA/pF. B: peak Cl⁻ currents deactivating at the holding voltage (I_DEACT) from Ano1₍₀₎ (left) or Ano1₍₀₎ΔEAVK (right), plotted vs. the conditioning voltage with 100–3,000 nM [Ca²⁺]_i (spectrum). C: I_DEACT from Ano1₍₀₎ (left) or Ano1₍₀₎ΔEAVK (right), plotted as a function of [Ca²⁺]_i after the indicated voltage steps (spectrum). EC₅₀ values (dashed lines): Ano1₍₀₎, 493 ± 9 nM; Ano1₍₀₎ΔEAVK, 761 ± 26 nM; n = 11, *P < 0.05, compared with Ano1₍₀₎ control by an unpaired t-test with Welch’s correction.