Fig. 5.

The Ano1₍₀₎ΔEAVK splice variant has slower activation kinetics than Ano1₍₀₎ at intermediate [Ca²⁺]_i. A: normalized Cl⁻ current traces activated by a step pulse to +80 mV from Ano1₍₀₎ (black) or Ano1₍₀₎ΔEAVK (gray) at 500 nM (left) or 3 µM (right) [Ca²⁺]_i. B: time constant of activation (τ_ACT) vs. voltage stimulus of Ano1₍₀₎ (black) or Ano1₍₀₎ΔEAVK (gray) with 500 nM (left) or 3 µM (right) [Ca²⁺]_i. P < 0.05, variation from EAVK. P > 0.05, variation from or interaction with voltage stimulus by a two-way ANOVA. *P < 0.05, by Bonferroni posttest. C: τ_ACT of Ano1₍₀₎ (black) or Ano1₍₀₎ΔEAVK (gray) vs. [Ca²⁺]_i. P < 0.05, variation from and interaction with [Ca²⁺]_i by a two-way ANOVA. *P < 0.05, by Bonferroni posttest; n = 5–16 cells per point.