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. 2017 Mar 17;312(6):H1120–H1127. doi: 10.1152/ajpheart.00851.2016

Fig. 1.

Fig. 1.

Effects of haptoglobin or hemopexin on the free hemoglobin-induced increase of systemic blood pressure in awake mice. Infusion of cell-free hemoglobin (Hb) but not albumin (Alb) increased systolic blood pressure (SBP) in awake mice (n = 8 mice/group). Haptoglobin (Hp) but not hemopexin (Hx) attenuated the Hb-induced increase in SBP. The increase (Δ) in SBP in mmHg (A) and results presented as the area under the curve (AUC) (B) are shown. *P < 0.05, Hb differed vs. Alb; #P < 0.05, Hb:Hx differed vs. Alb; †P < 0.05, Hb:Hp differed vs. Hb. Urine Hb concentration was increased in urine of wild-type mice collected at 1 h after infusion of free Hb or free Hb and Hx but not after infusion of free Hb and Hp (C). Infusion of Hb was associated with an increase in plasma Hb measured at 1 h after infusion. Plasma Hb was markedly increased in animals that received coinfusion of Hp (D). Representative plasma samples obtained at 1 h after injection shown at the bottom. There was a correlation between plasma nitric oxide (NO) consumption and plasma Hb levels at 1 h after injection (E and F). Numbers of mice (n) in each treatment group were as follows: Alb (n = 4), Hb (n = 11), Hb:Hp (n = 7), and Hb:Hx (n = 9). *P < 0.05 vs. Alb, #P < 0.05 vs. Hb. Data represent means ± SE.