Table 1.

ANCOVA of various flux values calculated based on the NMR spectra and O₂ consumption measurements

Comparison	P Value
Glucose flux
Control vs. 0.25 mM	0.0129
Control vs. 1 mM	0.0005
Control vs. 3 mM	0.0004
Control vs. 6 mM	0.0007
Fatty acid flux
Control vs. 0.25 mM	0.2216
Control vs. 1 mM	0.0038
Control vs. 3 mM	0.0025
Control vs. 6 mM	0.0059
Pyruvate dehydrogenase flux
Control vs. 0.25 mM	0.0129
Control vs. 1 mM	0.0005
Control vs. 3 mM	0.0004
Control vs. 6 mM	0.0007
Propionate anaplerosis
Control vs. 0.25 mM	0.0014
Control vs. 1 mM	0.0001
Control vs. 3 mM	0.0003
Control vs. 6 mM	0.0020
Total tricarboxylic acid flux
Control vs. 0.25 mM	0.5501
Control vs. 1 mM	0.2580
Control vs. 3 mM	0.1957
Control vs. 6 mM	0.1556

Group (propionate treatment) and O₂ consumption values were the variables used in the model. Numbers of mice for each group were as follows: 9 (control, 0.25 mM, and 1 mM), 8 (3 mM), and 7 (6 mM). ANCOVA, analysis of covariance.