Fig. 5.

Immunofluorescence demonstrates CFTR-dependent differences in the intracellular localization of SLC26A9. HEK293 cells were cotransfected with myc-SLC26A9 and either wt-GFP-CFTR (A–D) or F508del-GFP-CFTR (E–H). Fixed and permeabilized cells were then immunolabeled with mouse anti-myc (to detect SLC26A9, B and F) and rabbit anti-CAL antibody (C and G). D: the overlay of wt CFTR (A), myc-SLC26A9 (B), and CAL (C) demonstrates colocalization between wt CFTR and myc-SLC26A9 in the plasma membrane region (yellow arrowheads), and in an intracellular compartment that overlaps with CAL (white arrowheads). H: the overlay of F508del CFTR (E), myc-SLC26A9 (F), and CAL (G) demonstrates that myc-SLC26A9 primarily colocalizes with CAL (white arrowheads), and that both F508del CFTR and myc-SLC26A9 are absent at the plasma membrane. Scale bar in D and H, 5 µm. Similar results were observed in 3 biological replicates.