Fig. 4.

Increased neutrophil chemotaxis in WWOX-silenced cells is dependent on IL-8 and c-Jun transcription factor activity. A549 cells grown in culture were transfected with either a single or dual WWOX- and c-Jun-silencing miR-expressing plasmid. A: representative Western blot for WWOX and c-Jun in cell lysates resolved by SDS-PAGE is shown. Note that since WWOX and c-Jun are of similar molecular weight, the bands for each are derived from separate gels that were run at the same time and that contained the same fractions of the same lysates. B: scatter plot illustrates the results of a neutrophil chemotaxis assay in n = 3 experiments. C–E: scatterplots depict measured concentrations of IL-8, IL-1β, and IL-6. F: results of an NF-kB assay on nuclear extracts. Results are shown as means ± SD in n = 3 independent experiments. *P < 0.05, compared with control except where brackets indicate another comparison by Student’s t-test.