Fig. 3.

Sirtuin expression in neonatal and adult dermal fibroblasts. Primary dermal fibroblasts were isolated, propagated for four or five passages in cell culture, and mRNA (A) and protein (B and C) levels for SIRT1 and SIRT7 were tested. Donors included five neonatal controls (○) and four adult healthy volunteers (gray circles). A: SIRT1 and SIRT7 mRNA levels normalized to 18S and further normalized to the average of all neonatal samples. B: SIRT1 and SIRT7 protein levels in two neonatal cultures (N samples, lanes 1 and 2) and two adult cultures (A samples, lanes 3 and 4). C: densities of SIRT bands in B normalized to β-actin and further normalized to the average of neonatal samples. Horizontal bars in A and C indicate average values. P values were calculated using the Student’s t-test.