Fig. 6.

Effect of SIRT7 silencing on collagen and α-SMA protein levels. A: normal adult lung fibroblasts from three healthy control donors (C1–C3) were transfected with scrambled control or SIRT7 siRNAs, as indicated, and Western blotting for collagen type I protein (COL1) performed after 48 or 72 h. B: α-SMA protein levels for two healthy control donors (C1, C2) 72 h after silencing with scrambled or SIRT7 siRNAs. Noncontiguous gels are demarcated by white spaces. C: immunofluorescent staining for α-SMA in fibroblasts from a healthy control donor (different from the two controls shown in B) 48 h after transfection with scrambled or SIRT7 siRNAs. Note the increases in collagen and α-SMA in SIRT7-silenced fibroblast cultures.