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Proximity ligation assay (PLA) demonstrating TRPV1 and SERCA2 colocalization in primary human ASM cells. A: positive (red dots) PLA (PLA; Duolink, Invitrogen) in primary human ASM cells performed using primary antibodies against TRPV1 and SERCA2. This demonstrates that the proteins exist within 40 nm of each other, suggesting that TRPV1 expression is in the SR membrane. B: negative control with primary antibodies omitted. Bar: 100 µm; n = 3.
