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. 2017 Jul 4;11:183. doi: 10.3389/fncel.2017.00183

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Copyright © 2017 Vastagh and Liposits.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunocytochemical localization of kisspeptin (KP), galanin (GAL), cholecystokinin (CCK) and tyrosine-hydroxylase (TH) in the antero-ventral periventricular/MPA of the proestrous mouse. The uppermost row illustrates the MPA and its subdivisions in three representative rostro-caudal planes (from left to right). Red triangle indicates the border lines of tissue area sampled for the real-time PCR study. Immunofluorescent detection of KP, galanin, CCK and TH is provided in the three, representative frontal planes. KP, CCK and TH appear in cell bodies within the AVPV and the periventricular nucleus (PE) in addition to neuronal processes. Galanin-immunoreactivity is not detectable at the level of perikarya in proestrous mouse, despite its intense occurrence in axons. aca, anterior commissure; AVPV, antero-ventral periventricular nucleus; MPOM, medial division of the medial preoptic nucleus; MPOL, lateral division of the medial preoptic nucleus; OVLT, vascular organ of lamina terminalis; 3V, 3rd ventricle. Scale bar: 200 μm.