Fig. 3.

Cd39 deletion results in decreased macrophage numbers in fatty streaks from chow diet-fed mice. A: Macrophage levels were evaluated by Mac3 immunohistochemistry on aortic sinuses (one section per animal; n = 8 ApoE KO and 10 DKO mice). B: VSMCs infiltration was evaluated by Smc α actin immunohistochemistry on aortic sinus (one section per animal; n = 8 ApoE KO and 9 DKO mice). C: Intra-lesion apoptosis was investigated by TUNEL assay on aortic sinus (one section per animal; n = 7 ApoE KO and 10 DKO mice). Representative images are shown (scale bar is 250 μm in (A) and (B), 100 μm in (C). Quantification data are presented as mean ± SEM of the area staining or the percentage staining of the total plaque area. Setting a color threshold in ImageJ identified positive staining. *P < 0.05.