Fig. 2.

Inhibition of the Gα_12/13/RhoA/ROCK pathway is involved in the anti-fibrotic effects of CYP2J2. A: Representative immunoblots of G12 (TPR), G12 (Total), G13 (TPR), and G13 (Total) in cardiac tissue. B: Quantitation of Gα₁₂ activity and Gα₁₃ activity by determining the ratio of G12 (TPR)/G12 (Total) and G13 (TPR)/G13 (Total), respectively. C: Representative immunoblot of RhoA (RBD) and RhoA in cardiac tissue. D: Quantitation of RhoA activity by the ratio between RhoA (RBD) and RhoA (Total). E: Representative immunoblot of p-MYPT and MYPT in cardiac tissue. F: ROCK activity was evaluated by the ratio between p-MYPT and MYPT. **P < 0.05 versus WT-saline; ^&P < 0.05 versus WT-Ang II (n = 10 for each group).