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. 2017 May 29;58(7):1338–1353. doi: 10.1194/jlr.M074229

Fig. 4.

Fig. 4.

Inhibition of the Gα12/13/RhoA/ROCK pathway is involved in the anti-fibrotic effects of EETs. A: Representative immunoblots of G12 (TPR), G12 (Total), G13 (TPR), and G13 (Total) in cardiac fibroblasts treated with various reagents for 5 min. B: Quantitation of Gα12 activity and Gα13 activity by determining the ratio of G12 (TPR)/G12 (Total) and G13 (TPR)/G13 (Total), respectively. C: Representative immunoblot of RhoA (RBD) and RhoA (Total) and quantitation of RhoA activity by the ratio between RhoA (RBD) and RhoA (Total) in cardiac fibroblasts treated with various reagents for 5 min. D: Representative immunoblot of p-MYPT and MYPT and quantitation of ROCK activity by the ratio between p-MYPT and MYPT in cardiac fibroblasts treated with various reagents for 5 min. **P < 0.05 versus DMSO; &P < 0.05 versus Ang II; #P < 0.05 versus Ang II + 11,12-EET (n = 5 for each experiment).