TABLE 1.

Eicosanoids levels in cardiac tissue measured by LC/MS/MS

	Saline		Ang II
	WT	αMHC-CYP2J2-Tr	WT	αMHC-CYP2J2-Tr
AA-CYP epoxygenase
5,6-EET	51.90 ± 2.33	172.20 ± 6.13a	39.78 ± 2.00a	160.93 ± 3.87a,b
8,9-EET	62.61 ± 2.54	247.40 ± 4.48a	50.34 ± 3.25a	235.53 ± 5.40a,b
11,12-EET	80.69 ± 4.51	248.81 ± 5.75a	67.79 ± 3.75a	244.57 ± 2.13a,b
14,15-EET	142.54 ± 5.32	360.52 ± 6.55a	127.50 ± 4.73a	344.88 ± 2.10a,b
5,6-DHET	0.78 ± 0.07	1.41 ± 0.08a	0.53 ± 0.03a	1.17 ± 0.08a,b
8,9-DHET	1.70 ± 0.06	6.01 ± 0.10a	1.52 ± 0.05	5.55 ± 0.13a,b
11,12-DHET	2.65 ± 0.04	6.38 ± 0.18a	2.55 ± 0.03	5.95 ± 0.23a,b
14,15-DHET	2.58 ± 0.16	6.99 ± 0.18a	2.04 ± 0.10a	7.20 ± 0.13a,b
LA-CYP epoxygenase
12,13-EpOME	80.03 ± 2.73	82.08 ± 3.28	76.76 ± 2.41	79.30 ± 3.51
9,10-EpOME	98.24 ± 2.07	95.00 ± 3.43	102.42 ± 2.55	96.12 ± 2.98
12,13-diHOME	46.81 ± 2.49	50.42 ± 2.84	51.22 ± 3.28	53.27 ± 2.46
9,10-diHOME	31.22 ± 2.25	36.26 ± 3.20	35.13 ± 3.04	34.65 ± 3.53
AA-CYP allylic-oxidase
9-HETE	148.75 ± 3.82	150.73 ± 3.56	187.66 ± 1.17a	192.27 ± 1.91a
11-HETE	105.75 ± 2.10	110.29 ± 2.10	143.18 ± 3.78a	148.46 ± 5.02a
AA-LOX
15-HETE	154.15 ± 1.20	155.96 ± 1.24	197.26 ± 1.87a	201.49 ± 2.24a
5-HETE	188.28 ± 1.22	190.48 ± 1.04	237.41 ± 2.71a	242.65 ± 2.65a
8-HETE	64.10 ± 1.51	65.20 ± 1.14	85.08 ± 1.55a	84.23 ± 1.32a
AA-CYP ω-hydrolase
16-HETE	4.55 ± 0.12	4.77 ± 0.13	4.95 ± 0.10	5.00 ± 0.22
17-HETE	1.48 ± 0.07	1.39 ± 0.08	1.59 ± 0.07	1.22 ± 0.05
18-HETE	1.56 ± 0.08	1.78 ± 0.09	1.70 ± 0.10	1.68 ± 0.09
19-HETE	1.77 ± 0.05	1.93 ± 0.13	2.01 ± 0.06	1.82 ± 0.03
20-HETE	0.48 ± 0.05	0.61 ± 0.06	1.63 ± 0.05a	1.53 ± 0.03a

Eicosanoid levels are in picograms per milligram. LC/MS/MS results revealed that EETs and DHETs in cardiac tissue were increased in αMHC-CYP2J2-Tr mice under both saline- and Ang II-infused condition (n = 5 for each group). LA, linoleic acid; EpOME, epoxy-12Z-octadecenoic acid; diHOME, dihydroxyoctadecenoic acid.

^aP < 0.05 versus WT-saline.

^bP < 0.05 versus WT-Ang II.