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S100B and RAGE colocalization in the lumbar spinal cord from SOD1G93A rats. Double immunofluorescence was performed with anti-S100B (green) and anti-RAGE (red) in lumbar spinal cord from wild-type (WT), presymptomatic (ps-SOD1G93A), and end-stage (es-SOD1G93A) rats. The images were acquired from grey (GM) and white matter (WM) at two magnifications (scale bars: 50 μm left columns and 20 μm right column). Merged panels also show To-Pro-3 nuclear staining (TP3, blue).
