Figure 4.

RAGE increase in GFAP-positive cells in the cervical spinal cord from SOD1^G93A end-stage rats. Double immunofluorescence was performed with anti-RAGE (red) and anti-S100B (green) in cervical spinal cord from wild-type (WT), presymptomatic (ps-SOD1^G93A), and end-stage (es-SOD1^G93A) rats. The images were acquired from grey (GM) and white matter (WM) at two magnifications (scale bars: 50 μm left columns and 20 μm right column). Merged panels also show To-Pro-3 nuclear staining (TP3, blue).