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. 2005 Jan 27;24(4):800–812. doi: 10.1038/sj.emboj.7600545

Figure 2.

Figure 2

Cluster analysis for repeat-associated histone lysine methylation states. ChIP of wt and Suv39h dn ES cell chromatin with antibodies detecting H3-K9, H3-K27 and H4-K20 mono-, di- and trimethylation. As controls, ChIP was also performed with H3-K4 trimethyl (an active mark) antibodies and for the tubulin gene. Purified DNA from enriched chromatin fragments was amplified by real-time PCR with the repeat-specific primer sets (see Figure 1B), and values are indicated as percentage precipitation relative to the input. The dashed line represents the threshold signal of 0.5%, above which significant enrichment for distinct histone lysine methylation states was scored.