Skip to main content
. 2005 Jan 27;24(4):800–812. doi: 10.1038/sj.emboj.7600545

Figure 4.

Figure 4

Elevated transcript levels for repetitive DNA elements in Suv39h dn ES cells. RT–PCR analysis for repeat-derived transcript levels in total RNA from wt and Suv39h dn ES cells (A) or from wt (J1) and Dnmt1-/- or Dnmt3ab-/- ES cells (B). cDNA was generated by random priming and then amplified with the repeat-specific primer sets (see Figure 1B). Linear range amplification of cDNA was calibrated by real-time PCR (between 22 and 27 cycles; see Supplementary Figure S2), and PCR products are visualized by inverse images of EtBr-stained 2% agarose gels. As controls, reactions were performed without reverse transcriptase (−RT). The relative average increase in transcription of two distinct elements within a repeat class is indicated in the middle of the two columns shown in panel A.