Figure 5.

Tandem satellite repeats generate dsRNA. Total RNA from wt and Suv39h dn ES cells was treated for the indicated time points with RNAseONE™ to digest ssRNAs (A) or with RNAseV1 to cleave dsRNAs (B). The remaining pool of RNA molecules was then converted to cDNA and amplified by real-time PCR with the repeat-specific primer sets (see Figure 1B). The histograms indicate the percentages, relative to undigested RNA, of repeat-derived RT–PCR products that can be generated after RNAseONE™ (% dsRNA) or RNAseV1 (% ssRNA) treatment.