Table 4.
Genetic diversity analysis with InDel markers developed in radish
| InDel Primer name | Scaffold | Position | Expected | Na | HO | HE | PIC | Forward primer | Reverse primer |
|---|---|---|---|---|---|---|---|---|---|
| size (bp) | |||||||||
| NAU-RsInDel1 | Rsa1.0_02158.1 | 133,381 | 124 | 2 | 0.27 | 0.36 | 0.38 | TCCAGCACCAAGAACTAT | TAAAACACCACCAAAAGG |
| NAU-RsInDel2 | Rsa1.0_04545.1 | 9016 | 154 | 2 | 0.27 | 0.51 | 0.46 | GTCGGATTTGGTAAAGAGG | CGTCCCTGAACTGGTCATA |
| NAU-RsInDel3 | Rsa1.0_00949.1 | 39,724 | 141 | 2 | 0.53 | 0.47 | 0.44 | CATCTTCGGGATCAACGG | ATCGGACAACTCAAAACCAACT |
| NAU-RsInDel4 | Rsa1.0_00251.1 | 287,457 | 180 | 2 | 0 | 0.5 | 0.49 | AGACTGCCGAGTATCAAT | AATCGCTGGAGAAGAAAT |
| NAU-RsInDel5 | Rsa1.0_03247.1 | 13,547 | 96 | 2 | 0.16 | 0.25 | 0.26 | AGTGTCTTTGCGGCATCT | TAGGGTTTGCGATTTGGA |
| NAU-RsInDel6 | Rsa1.0_00071.1 | 141,781 | 111 | 2 | 0.25 | 0.5 | 0.51 | TGAGGAAACAGAACAAGA | GCTTAGGCTTAGCATTAT |
| NAU-RsInDel7 | Rsa1.0_00660.1 | 52,677 | 175 | 2 | 0.16 | 0.43 | 0.38 | TGCTATCAAAACGCAAAT | CAAGGAAAATAAAACTATGGAG |
| NAU-RsInDel8 | Rsa1.0_01209.1 | 39,624 | 112 | 2 | 0.07 | 0.43 | 0.41 | ATCATCAATCTCGCTCTTT | AGACTATCACCTCCTCTGC |
| NAU-RsInDel9 | Rsa1.0_01100.1 | 52,433 | 153 | 2 | 0.31 | 0.35 | 0.28 | AGCAGCCAGTGAGATTAG | ACACGGTATTTTCCAACC |
| NAU-RsInDel10 | Rsa1.0_00232.1 | 38,667 | 162 | 2 | 0.52 | 0.51 | 0.42 | TAGTGAGTGAAGACACCAAC | TTAAAGCTGCAGAAGAAG |
| NAU-RsInDel11 | Rsa1.0_01156.1 | 25,754 | 165 | 2 | 0.1 | 0.34 | 0.32 | CCCTCTGCTTTCATCCTT | TCTCCGCTGACCACAATT |
| NAU-RsInDel12 | Rsa1.0_15591.1 | 936 | 140 | 2 | 0.39 | 0.39 | 0.35 | TACTCCAAATTCGCATCA | TCATCGGAAGGTAAGACG |
| NAU-RsInDel13 | Rsa1.0_01184.1 | 26,764 | 177 | 2 | 0.07 | 0.29 | 0.37 | ATGATTCTCCATCCAAGC | GTCGCATTTTCATTTCCT |
| NAU-RsInDel14 | Rsa1.0_03334.1 | 18,880 | 127 | 4 | 0.41 | 0.75 | 0.69 | CTACTTGTCCGAGCTTCA | TAACTCCCGTTGTTGATA |
| NAU-RsInDel15 | Rsa1.0_27069.1 | 235 | 109 | 2 | 0.25 | 0.31 | 0.26 | TTATGAGAAACGTCAAGG | AGTGGAGAAAGGAACAAG |
| NAU-RsInDel16 | Rsa1.0_20672.1 | 478 | 172 | 3 | 0.19 | 0.51 | 0.45 | TAGAGGGTGAAACGCAGGAA | TTGGGAAGTGGAACAAAGCA |
| NAU-RsInDel17 | Rsa1.0_00250.1 | 39,440 | 164 | 2 | 0.19 | 0.36 | 0.33 | ATTTCAACAGATAAACCGACAC | GCCTGAGCCCATCAACTA |
| NAU-RsInDel18 | Rsa1.0_02411.1 | 6272 | 178 | 2 | 0.06 | 0.48 | 0.41 | GGGAACCCAACAACCTAT | TTATTCGACGGCACAAAC |
| NAU-RsInDel19 | Rsa1.0_00016.1 | 354,003 | 162 | 2 | 0.46 | 0.46 | 0.53 | TCCAAGGCTAGTAGAAAC | TGACATCCAACAAAGACA |
| NAU-RsInDel20 | Rsa1.0_01401.1 | 93 | 134 | 2 | 0.34 | 0.47 | 0.35 | AAATAAGACACGAAACCCTG | ATCGAAACTCCTCCCTCC |
| NAU-RsInDel21 | Rsa1.0_01219.1 | 21,510 | 148 | 2 | 0.13 | 0.5 | 0.45 | ACAACAACGGAGACTTGG | GTGAACCTGTCGGCTATG |
| NAU-RsInDel22 | Rsa1.0_00816.1 | 110,085 | 145 | 2 | 0.17 | 0.48 | 0.44 | GTCCGAACAAAAGGTGAGATT | ACTATTGATTTGATTTGGGTTGTG |
| NAU-RsInDel23 | Rsa1.0_00744.1 | 53,460 | 145 | 2 | 0.37 | 0.44 | 0.42 | TCTCAAGGACCCAACACTACA | TTGCAAAACTGGAAAAGGAA |
| NAU-RsInDel24 | Rsa1.0_02097.1 | 17,223 | 124 | 2 | 0.19 | 0.42 | 0.37 | TAAAATTGATAAACCTGCTC | CTGTGATTCTGAACCTCC |
| NAU-RsInDel25 | Rsa1.0_01975.1 | 14,276 | 163 | 2 | 0.03 | 0.03 | 0.09 | CCAGAAAACGAATGAAAAACTACT | AGAAACACATGGCTGAGAGGA |
| NAU-RsInDel26 | Rsa1.0_00101.1 | 211,799 | 180 | 2 | 0.34 | 0.5 | 0.37 | CAGCGAAACCACAAACACAA | GCTAAGTCTCCCTCCTCCATT |
| NAU-RsInDel27 | Rsa1.0_04806.1 | 7466 | 184 | 2 | 0.31 | 0.41 | 0.32 | TGTATTAACCACCACCAT | CACGAAAGATAACAACTCAA |
| NAU-RsInDel28 | Rsa1.0_04167.1 | 18,003 | 200 | 2 | 0.19 | 0.35 | 0.28 | TCTCACCCCAGCCACTTT | TATCCCACCTGTCTCGTT |
| NAU-RsInDel29 | Rsa1.0_02690.1 | 16,462 | 165 | 2 | 0.25 | 0.51 | 0.38 | AATCTTCCTGCGCCACAT | TCGTTTGCCTTCTCCTCA |
| NAU-RsInDel30 | Rsa1.0_08988.1 | 3062 | 105 | 2 | 0.23 | 0.49 | 0.41 | AGAGGGAAGCCACTATCA | TTCCGAAGATTAGATGAGAT |
| NAU-RsInDel31 | Rsa1.0_14009.1 | 2697 | 189 | 3 | 0.26 | 0.46 | 0.42 | TCTTGTTCTTTGGGTAAT | AAGGACTCAATCTATGGC |
| NAU-RsInDel32 | Rsa1.0_02642.1 | 14,660 | 104 | 3 | 0.03 | 0.48 | 0.5 | TACAATAAAACCCTCAAG | AAGTGGTGATAAGGAAAC |
| NAU-RsInDel33 | Rsa1.0_02467.1 | 15,972 | 131 | 2 | 0.03 | 0.5 | 0.45 | CAGATAAAGCCAAAAGTC | ATCTGTTTTACTTGGAGC |
| NAU-RsInDel34 | Rsa1.0_02467.1 | 15,842 | 130 | 3 | 0.28 | 0.57 | 0.49 | TCTCAAACCAAGGAAACC | ACGAAGAACTACGAGGCT |
| NAU-RsInDel35 | Rsa1.0_00012.1 | 70,110 | 193 | 2 | 0.41 | 0.5 | 0.37 | TTTTCGAGAAGAAGAAAGTC | GGACAAATACTGCCACAT |
| NAU-RsInDel36 | Rsa1.0_00042.1 | 55,669 | 124 | 2 | 0.28 | 0.48 | 0.36 | TAAAAGCCAATACAGAAG | CACAGACGAAAGACCATA |
| NAU-RsInDel37 | Rsa1.0_00105.1 | 127,102 | 105 | 2 | 0.29 | 0.5 | 0.41 | GGAGGAAGACGAGTTGAT | TGAGACGTTTTGGAAGTG |
| NAU-RsInDel38 | Rsa1.0_01482.1 | 15,564 | 102 | 2 | 0.26 | 0.41 | 0.49 | TAAAATAACAAATCACTCCCTC | CTAACCCTAATCGCACCC |
| NAU-RsInDel39 | Rsa1.0_00816.1 | 109,998 | 140 | 2 | 0.25 | 0.51 | 0.38 | CTCACAACCCAAATCAAA | TACCTGGAGGAAGGAAAG |
| NAU-RsInDel40 | Rsa1.0_04618.1 | 3100 | 117 | 2 | 0.47 | 0.49 | 0.45 | GAAGAGCTGGAGAAGGAA | AGGCCAACTGCTAGTAGATT |