Fig. 1. Modification of TEM β-lactamase. The structure of TEM β-lactamase (top left) with the inhibitor imipenem (yellow sticks to emphasise the substrate binding pocket) highlights the residues targeted for replacement with azF (red spheres) together with the key catalytic residues (cyan sticks) and the Ω-loop (green). The protein structures were generated using PyMol.16 A representation of the SPAAC reaction using the activated alkyne present in the core DBCO moiety is shown (top right). The R groups attached to the DBCO used in this study are shown (bottom half).
