Figure 6.

Lip1p is an integral ER membrane protein. The strains used were RH 5666 and RH 6068. (A) Cell lysates were treated with different chemical reagents as indicated, centrifuged, and pellet (P) and soluble (S) fractions were analyzed by Western blotting. (B) Cell homogenates were separated on sucrose density gradients. Fractions were collected from the top of the tube and analyzed by Western blotting. Wbp1p is a marker of the ER, Emp47p of the Golgi apparatus. (C) Colocalization of Kar2p and Lip1p by indirect immunofluorescence (upper panels), additional merged images with DAPI (middle panels). Immunofluorescence of Flag-Lac1p and Flag-Lip1p (lower panels).