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Lip1p topology. (A) Microsomal membranes from strains RH6068, RH5666 or RH6348 were incubated with or without Triton X-100 in the presence or absence of Proteinase K. The samples were analyzed by Western blotting to reveal the Flag tag and Kar2p. (B) Flag-Lip1p-ΔNT (RH6069) was visualized by indirect immunofluorescence. (C) Cells (strains RH4838, RH5309, RH6013 and RH6069) were labelled with [3H]DHS and analyzed as in Figure 8.
