Figure 1.

(a) Polymerase chain reaction amplification picture of cefotaximase gene in extended-spectrum beta lactamase producing Escherichia coli strains after agarose (1.5%) gel electrophoresis. Lane M: Molecular marker 100 bp DNA ladder; lane 1: Positive control; lane 2: Negative control; lane 3 and 4: Escherichia coli clinical isolates. (b) Polymerase chain reaction amplification of temoneira gene. Lane M: 100 bp DNA ladder; lane 1: Positive control; lane 2–6: Escherichia coli clinical isolate; lane 7: Negative control. (c) Polymerase chain reaction amplification of sulfhydryl variable gene. Lane M: 500 bp DNA ladder; lane 1: Positive control; lane 2: Negative control; lane 3 and 4: Escherichia coli clinical isolates