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. 2017 Jul 4;8:156. doi: 10.1186/s13287-017-0606-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Differentiation efficiency of male and female hPSCs into smooth muscle progenitor cells (pSMCs). a Schematic of the protocol for differentiation of hPSCs to pSMCs and terminal differentiated SMCs. b Percentage of CD34⁺/CD31⁺ cells in the male and female hPSC derivatives. All hPSCs showed similar differentiation efficiency via a standardized differentiation protocol. D day, CDM chemically defined media, ABC Activin A, bone morphogenetic protein 4, and CHIR99021, FGF fibroblast growth factor, VEGF vascular endothelial growth factor, SMGS smooth muscle growth medium, SMDS smooth muscle differentiation medium, iPSC induced pluripotent stem cell, ESC embryonic stem cell